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4.1. INTRODUCTION Human embryonic stem cells (hESCs) have the potential to differentiate to cardiomyocytes in culture. They are easily identi able rst as areas of rhythmically contracting cell clumps. Further detailed electrophysiological and immunohistochemical analysis shows that these clumps contain individual cardiac cells with a spectrum of phenotypes comparable to those found in the normal human fetal heart at 8 10 weeks of gestation [reviewed in Boheler et al., 2002; Passier and Mummery, 2005]. They are therefore of interest for studying differentiation of cells during early human heart development, as well determining the physiological and pharmacological properties of human cardiomyocytes, including their responses to cardiac drugs. At some point in the future, they may also represent a source of transplantable cells for cardiac muscle repair by replacement of cardiomyocytes lost during ischemic damage. The differentiation protocols that are effective in inducing hESCs to differentiate to cardiomyocytes depend in part on the individual hESC cell line used and in part on the methods used for propagation before differentiation. Here several methods for generating and characterizing cardiomyocytes from hESCs are described, as well as methods for dissociation of cardiomyocytes into single-cell suspensions that are useful for both characterizing cells by antibody staining and electrophysiological measurements as well as preparing cells for transplantation into (animal) hearts.

ter of mass, 5 .

6. Add a private function named loginHandler() that receives an event argument typed as LoginEvent and returns void. 7. Within the private function, save the event object s login property to the bindable myLogin variable:

Next, you provide the sales projection numbers in column B. Assume that January s sales are projected to be $50,000, and that sales will increase by 3.5 percent in each of the subsequent months.

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public interface Sensoruser extends MsgHandler { void globalPredicateTrue ( i n t G [ ] ) ; void g l o b a l P r e d i c a t e F a l s e ( i n t pid ) ;

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to be rendered verbatim. For example, the following text will be spaced exactly as shown in the following code:

angle of incidence The angle at which light rays strike a surface angle of reflection The angle at which light rays reflect from a surface The angle of reflection equals the angle of incidence The angle of reflection applies only to a specular reflection where the surface is uniform In a diffused reflection, light is reflected in all different angles aperture The lens opening that lets in light The diameter of this opening, in addition to the focal length, determines the f-stop number The f-stop number is sometimes referred to as the aperture number, or aperture for short The f-number is the focal length divided by the aperture diameter Both units are in millimeters and the resulting f-number is unit-less aspherical lens A lens that is not spherical in shape.

In this new function definition for emailEntries(), things look an awful lot like the previous emailAggregatorPage() function. In fact, if you re careful, you could copy and paste much of it over. This function starts off with a parameter list and a docstring, and begins iterating through the entries passed to it. For each entry, it builds a new subject line by filling in a subject header template passed in as a parameter. Then, it creates the MIME-Multipart message shell with the usual parts, including the To and From email addresses, the subject line, and the throwaway preamble and epilogue values. And, as in the previous email code, it builds and attaches a simple plain text message part referring to the HTML content in the next attachment. Now, you wrap up this function by borrowing a little code from writeAggregatorPage() to generate the HTML content for this email, as shown in Listing 3-19.

As mentioned throughout this book, buttons such as those used in the menu examples gain their functionality by having ScriptMaker scripts or a script step attached to them. In the following series of steps, we explain how to design a navigation menu like the one used by the New Buttons database (refer to Figure 23-1).